The present study aimed to estimate the effect of salicylic acid (400 mg L-1) and sodium nitroprusside (150 mg L-1) and their interaction on canola plants. under two water stress level at (50, and 100% F.C). This experiment was carried out at the Experimental Farm, Faculty of Agriculture, Suez Canal University, Ismailia, during growing season of 2020/21. The obtained results indicated that increasing water stress levels from 100% to 50% F.C caused significant decrease in all studied growth (except root length) and yield characters, photosynthetic pigments, relative water content (RWC) and some anatomical characters of root and leaves. Also, results showed that salicylic acid and sodium nitroprusside tested treatments statistically improved growth parameters, and the studied anatomical characters. Conclusively, this study indicates that, foliar application with salicylic acid and sodium nitroprusside and their interaction can decrease the effects of drought stress on growth and yield of Canola (Brassica napus L.).