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418887

Occurrence and Molecular Characterization of Newcastle Disease Virus Strains in Chicken Flocks in Ibadan, Nigeria: Implications for Vaccine Strain Compatibility

Article

Last updated: 09 Apr 2025

OverviewSimilar Items

Subjects

-

Tags

Poultry diseases

Abstract

Newcastle Disease (ND) poses a significant threat to poultry health and economic stability in Nigeria, with outbreaks affecting both smallholder and commercial farms. Despite vaccination efforts, the persistence of ND outbreaks is attributed to the genetic diversity of circulating Newcastle disease virus strains, which often differ from vaccine strains. This study investigated the occurrence and molecular characteristics of ND virus in chicken flocks within Ibadan between June and September 2024. A cross-sectional study was conducted by collecting samples from chickens that were tentatively diagnosed as ND at Poultry Diagnostic Centers in Ibadan. The presence of ND virus was confirmed through haemagglutination and haemagglutination inhibition tests, followed by molecular analysis using reverse transcription-polymerase chain reaction (RT-PCR) targeting the F-gene. Genetic sequencing and phylogenetic analysis assessed the relationship between detected strains and available vaccine strains. Out of 297 poultry cases submitted for diagnostic evaluation, 26 were suspected to be ND based on clinical signs and pathological findings, yielding an occurrence rate of 8.8%. Following haemagglutination and haemagglutination inhibition tests using ND-specific antiserum, 12 samples (4.04%) tested positive for ND virus. At the same time, PCR analysis confirmed the presence of the ND virus in 11 out of the 12 cases, with strains showing high genetic similarity to local field strains but notable variations from existing vaccine strains. The analysis indicated that the strains belonged to the avian paramyxovirus 1 lineage, with a sequence identity of 99.63% among them. The study highlights the need for ongoing molecular surveillance of ND virus in Nigeria to guide vaccination strategies considering the genetic diversity of circulating strains.

DOI

10.21608/javs.2025.363445.1543

Keywords

Genetic diversity, Molecular characterization, Newcastle Disease virus, Nigeria, RT-PCR, Vaccination

Authors

First Name

Aminata

Last Name

BALDE

MiddleName

-

Affiliation

Pan African University Life and Earth Sciences Institute Including (Health and Agriculture), Ibadan, Nigeria

Email

aminatabalde1122@gmail.com

City

-

Orcid

0009-0004-1741-8742

First Name

Oladele

Last Name

Omolade

MiddleName

Abodunrin

Affiliation

Pan African University Life and Earth Sciences Institute Including (Health and Agriculture), Ibadan, Nigeria; Department of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria

Email

dupelade24@gmail.com

City

Ibadan

Orcid

-

First Name

Bakre

Last Name

Azizat

MiddleName

Adetolase

Affiliation

Department of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria

Email

bakreadetolase@gmail.com

City

-

Orcid

-

First Name

ONIDJE

Last Name

EDMOND

MiddleName

-

Affiliation

Pan African University Life and Earth Sciences Institute (including Health and Agriculture), Ibadan, Nigeria

Email

eonimond@gmail.com

City

IBADAN

Orcid

0009-0000-1876-6694

First Name

Eyarefe

Last Name

David

MiddleName

-

Affiliation

Department of Veterinary Surgery and Radiology, University of Ibadan, Ibadan, Nigeria; Department of Veterinary Surgery and Radiology, University of Ibadan, Ibadan, Nigeria

Email

od.eyarefe@gmail.com

City

-

Orcid

-

First Name

Igado

Last Name

Olawumi

MiddleName

Olumayowa

Affiliation

Pan African University Life and Earth Sciences Institute Including (Health and Agriculture), Ibadan, Nigeria; Department of Veterinary Anatomy, University of Ibadan, Ibadan, Nigeria

Email

mayowaigado@yahoo.com

City

Ibadan

Orcid

-

Volume

10

Article Issue

2

Related Issue

54805

Issue Date

2025-04-01

Receive Date

2025-02-24

Publish Date

2025-04-01

Page Start

128

Page End

136

Print ISSN

1687-4072

Online ISSN

2090-3308

Article File

JAVS_Volume 10_Issue 2_Pages 128-136.pdf

PDF . 643.8kB

Link

https://javs.journals.ekb.eg/article_418887.html

Detail API

http://journals.ekb.eg?_action=service&article_code=418887

Order

14

Type

Original Article

Type Code

1,095

Publication Type

Journal

Publication Title

Journal of Applied Veterinary Sciences

Publication Link

https://javs.journals.ekb.eg/

MainTitle

Occurrence and Molecular Characterization of Newcastle Disease Virus Strains in Chicken Flocks in Ibadan, Nigeria: Implications for Vaccine Strain Compatibility

Details

Type

Article

Created At

09 Apr 2025