The objective of this study was to examine the correlation between the maternal antibody titers (MAT) on the Newcastle disease virus (NDV) and infectious bursal disease virus (IBDV), as well as various hematological and biochemical parameters, in five groups of one-day-old chicks. Following hatching, a total of 150 chicks were acquired, with 30 chicks obtained from each line. These chicks were not vaccinated and were subsequently divided into five groups, namely T1 representing black (BK), T2 representing nicked neck (NK), T3 representing bard (BD), T4 representing brown (BN), and T5 representing white (WT). The outcomes were analyzed statistically among all groups (P < 0.05). The results showed an increase in MAT against NDV was highest in WT group (13.649), while the decrease was the lowest in BK group (7.731). BK and BN recorded superior value in GOT concentration (21.0, 19.0, respectively). Group 3 (BD) demonstrated the most significant enhancements in MAT against IBDV, with a value of 10.833. This was followed by BK, WT, BN, and NK, which recorded values of 7.429, 6.852, 5.395, and 2.781, respectively. However, the various groups showed no significant variations in blood composition, except for heterophil, which exhibited significant differences across all groups. The values of MAT that were obtained from BK, WT, and BN were considered to be the most optimal, with respective measurements of 16.0, 19.0, and 20.0. The results found in this study indicated that there was a potential for genetic lines to influence the properties of maternal antibodies against NDV, IBDV, and other hematological parameters.