Background: Food handlers could be a source of nosocomial foodborne outbreaks since they could contaminate food at any point from purchase to distribution. It is essential to ensure that food handlers receive training on safe food handling practices.  Aim: To evaluate the effect of health education for food handlers about food safety in Port Said hospitals. Subjects and Method: A quasi-experimental design was used. Subjects: A purposive sample of 59 food handlers dealing with food production, preparation, and storage in hospital's kitchens. Setting: The study conducted at all hospitalists' kitchens whichaffiliated to the healthcare hospitals in Port said. Tools: Three tools were used for data collection; the first tool Food Handlers questionnaire, the second tool Food Safety Attitude Point Rating Scale, the third tool Observational food handlers' practice checklist. In addition to the personal and sociodemographic data sheet. The Results: 69.5% of the food handlers had good knowledge level post-program compared to 13.6% pre-program. 76.3% of them had positive attitude level post-program compared to 44.1% pre-program. 67.8% of them had good practice level post-program compared to 16.9% pre-program. in addition, there was statical significant positive correlation between the food handlers' knowledge, attitude, and practice post-program. Conclusion: The health educational program effect positively on food handlers' knowledge, attitude, and practice about food safety. Recommendations: Design additional studies about safe food handling for food handlers in other hospitals in Egypt.