Background: Acute pancreatitis is a common cause of acute abdomen presenting to the emergency. It can mimic most cases of acute abdomen clinically like cholecystitis, perforated duodenal ulcer etc. Acute pancreatitis accounts for 5% of patients presenting with abdominal pain to emergency. This study aimed to investigate the predictive role of Urinary Trypsinogen-2 (UT-2) dipstick test in early diagnosis of acute pancreatitis in comparison with serum amylase and lipase levels. Methods: This cross-sectional study was carried out on 104 patients who were suspected to have acute pancreatitis. Patients were categorized into two groups: Group I: Patients with acute pancreatitis (have elevated lipase and amylase level (above 300 IU/L in serum) and diagnostic findings on contrast-enhanced computed tomography (CECT). Group II: Patients with other abdominal disorders. Blood samples were analyzed for serum amylase, lipase, and UT-2 dipstick, while CECT confirmed diagnoses. Results: Of the patients, 12 (11.54%) were diagnosed with acute pancreatitis. UT-2 was significantly higher in group I than group II (P