ManageChatDiscover
421068

Neonatal sepsis; A New Paradigm in Laboratory Diagnosis

Article

Last updated: 09 Apr 2025

OverviewSimilar Items

Subjects

-

Tags

Clinical microbiology

Abstract

Neonatal sepsis is a leading cause of morbidity and mortality worldwide, necessitating rapid and accurate diagnostic methods to initiate proper treatment. Traditional blood cultures, while considered the gold standard for diagnosing sepsis, have limitations such as low sensitivity and prolonged time to results, which can delay critical therapeutic interventions. Various biomarkers such as C-reactive protein (CRP), procalcitonin (PCT) and interleukin-8 are used for diagnosis of sepsis. However, these biomarkers may also be elevated in noninfectious conditions such as premature rupture of membranes, fetal distress and perinatal asphyxia, resulting in false positive results and low specificity for neonatal sepsis The amplification of the 16S ribosomal RNA (rRNA) gene by polymerase chain reaction (PCR) has emerged as a promising alternative due to its potential for rapid and sensitive detection of bacterial pathogens. The diagnostic test must be rapid and sensitive to improve the outcome associated with neonatal sepsis  and to avoid over use of antibiotics.

DOI

10.21608/ejmm.2025.371856.1539

Keywords

16s rRNA gene, automated blood culture, PCR sensitivity, C-Reactive protein (CRP), Diagnostic Biomarkers

Authors

First Name

Nourhan

Last Name

Mostafa

MiddleName

A.M.

Affiliation

Resident of Pediatrics, Ministry of Health, Egypt

Email

nooormostafa33@gmail.com

City

-

Orcid

-

First Name

Sohier

Last Name

Abdelrazek

MiddleName

Y.

Affiliation

Pediatrics Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt

Email

sohier_yahia@yahoo.com

City

-

Orcid

-

First Name

Waleed

Last Name

Eldars

MiddleName

M.M.

Affiliation

Medical Microbiology and immunology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt

Email

wellydars@gmail.com

City

-

Orcid

0000-0002-4256-4977

First Name

Ahmed

Last Name

Eita

MiddleName

M.

Affiliation

Pediatrics Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt

Email

mahfo2005@hotmail.com

City

-

Orcid

-

Volume

34

Article Issue

4

Related Issue

54773

Issue Date

2025-10-01

Receive Date

2025-03-27

Publish Date

2025-10-01

Print ISSN

1110-2179

Online ISSN

2537-0979

Link

https://ejmm.journals.ekb.eg/article_421068.html

Detail API

http://journals.ekb.eg?_action=service&article_code=421068

Order

421,068

Type

Review articles

Type Code

2,039

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Neonatal sepsis; A New Paradigm in Laboratory Diagnosis

Details

Type

Article

Created At

09 Apr 2025