The objective of this study was to assess bioactive compounds and antibacterial properties of Palmaria palmata algae extract against some pathogenic bacteria. Following gas chromatography (GC-MS), the chemical content of the methanolic extract of the Palmaria palmata sample was determined. The antibacterial activity was tested against Staphylococcus aureus, Bacillus cereus, Proteus mirabilis, and Pseudomonas aeruginosa using the paper disc method. Regardless of the Palmaria palmata algae extract, GC-MS analysis enabled the identification of sixteen chemical compounds. The results showed that Palmaria extract exhibited significant antibacterial activity against Staphylococcus aureus, Bacillus cereus, Proteus mirabilis and Pseudomonas aeruginosa, which are common and complex pathogens. Our research contributes to the suggestion that Palmaria palmata algae extract have bioactive compounds and antibacterial activity and Palmaria extracts have four pharmacological applications.