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420866

Levels of Co-inhibitory Receptors TIM- 3, LAG-3 and TIGIT in Preeclampsia Patients

Article

Last updated: 09 Apr 2025

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-

Tags

Clinical microbiology

Abstract

Background: Preeclampsia (PE) is a hypertensive disorder that affects 2-15% of pregnancies and is linked to immune system overactivation. Co-inhibitory receptors play a key role in regulating the immune system during pregnancy. Objective: This study aimed to compare expression levels of co-inhibitory receptors LAG-3, TIM-3 and TIGIT in blood and decidual tissue, as well as the serum IL10 levels between preeclampsia patients and those with normal pregnancy and to assess their diagnostic potential in predicting preeclampsia including those with severe features. Methodology: Blood and decidual tissue samples were collected from 24 patients with PE and 24 healthy pregnant controls. Expression of TIM-3, TIGIT and LAG-3 genes was determined by RT-qPCR. Serum IL-10 levels were measured by ELISA in both groups. Results: our results showed significantly reduced expression in blood TIM-3, LAG-3 and TIGIT as well as decidual LAG-3 and TIGIT among PE patients compared to controls. The decrease in expression levels was significantly pronounced in the blood than in the decidua of PE patients. Serum levels of regulatory cytokine IL10 were also significantly lower in PE cases compared to controls, with a significant positive correlation with tissue TIM-3. Additionally, TIGIT expression was significantly lower in PE patients with severe features compared to those without. Conclusions: The expression levels of immune checkpoints (TIM-3, LAG-3, and TIGIT) and IL-10 in the blood and decidua of patients with preeclampsia (PE) differ from those in healthy pregnancies, indicating their potential role in the pathogenesis and prediction of PE and its complications.

DOI

10.21608/ejmm.2025.371652.1535

Keywords

preeclampsia, co-inhibitory receptor, LAG‐3, TIGIT, TIM‐3

Authors

First Name

Zeinab

Last Name

Sayed

MiddleName

I.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University

Email

zeinababdelfadeel155@gmail.com

City

-

Orcid

-

First Name

Noha

Last Name

Afifi

MiddleName

A.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University

Email

nohaafifi2015@aun.edu.eg

City

Assiut

Orcid

-

First Name

Nahla

Last Name

Elsherbiny

MiddleName

M.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University

Email

nahlaelsherbiny@aun.edu.eg

City

-

Orcid

-

First Name

Reda

Last Name

Hussein

MiddleName

s.

Affiliation

Department of Obstetrics and Gynecology, Faculty of Medicine, Assiut University

Email

rsalah313@aun.edu.eg

City

-

Orcid

-

First Name

Asmaa

Last Name

Shaltout

MiddleName

S.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University

Email

asmaashaltout@aun.edu.eg

City

Assiut

Orcid

-

Volume

34

Article Issue

3

Related Issue

53640

Issue Date

2025-07-01

Receive Date

2025-03-27

Publish Date

2025-07-01

Print ISSN

1110-2179

Online ISSN

2537-0979

Link

https://ejmm.journals.ekb.eg/article_420866.html

Detail API

http://journals.ekb.eg?_action=service&article_code=420866

Order

420,866

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Levels of Co-inhibitory Receptors TIM- 3, LAG-3 and TIGIT in Preeclampsia Patients

Details

Type

Article

Created At

09 Apr 2025