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420367

Polymorphism Effects of TLR2 and TLR4 genes on Different Otitis Media Bacterial Infections

Article

Last updated: 09 Apr 2025

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Tags

Clinical microbiology

Abstract

Background: Otitis media (OM) is an infection or inflammation in the middle ear. OM can become chronic or cause difficulties if it is not recognized early in the middle ear or if sufficient immune response is not elicited. Objective: We investigated the role of SNP of Toll-like receptors (TLRs) in otitis media because of their importance in the early response to external antigens. Methodology: A case-control study was carried out from 27/9/2020 to 25/2/2021 on 120 Iraqi OM patients and 80 controls. They were collected from 50 females and 70 males with different ages ranging from 5 and 68 years. The VITEK 2 system was used to analyze bacterial isolates and identify bacterial species. For the extraction and purification of genomic DNA from blood samples, the Gene aid gSYNCTM DNA extraction kit fast technique. Results: (83.3 %) of patient swabs yielded positive cultures, indicating otitis media, and (16.7 %) yielded negative cultures. Two variants (SNPs: rs5743708 and rs121917864) of the TLR2 gene were investigated. The SNP rs5743708 allele and genotype frequencies differed significantly between patients' group and controls (p-values = 0.0002, 0.0203, and 0.0245,) respectively. Comparing Otitis media patients to control showed that the SNP rs121917864 had a similar allele and genotype frequencies distribution, and the p-values were insignificant. Two variants (SNPs: rs4986790 and rs4986791) of the TLR4 gene were investigated. Conclusion: There were a significant differences (p-value = 0.0008, 0.0109, and 0.0146) in the TLR4 gene SNP rs4986790 distribution of allele and genotype frequencies among patients to controls. However, when comparing patients to controls, significant differences in the distribution of allele and genotype frequencies of SNP rs4986791 were discovered (p-value = 0.0001, 0.0022, and 0.0318). In this study, SNPs (TLR2 gene: rs5743708; TLR4 gene: rs4986790 and rs4986791) were significantly associated with susceptibility to otitis media.

DOI

10.21608/ejmm.2025.362574.1493

Keywords

Keywords: Otitis media, SNP, TLR2, TLR4, bacterial infection

Authors

First Name

Rafal

Last Name

Enad

MiddleName

-

Affiliation

Ministry of Health, Anbar Health Directorate, Iraq

Email

-

City

-

Orcid

-

First Name

Abdulla

Last Name

Khalaf

MiddleName

H.

Affiliation

Department of Medical Laboratories Techniques, College of Health and Medical Techniques University Of Al-Maarif , Al Anbar,31001, Iraq

Email

-

City

-

Orcid

-

First Name

Ahmed

Last Name

Sulaiman

MiddleName

A.

Affiliation

Department of Biotechnology, College of Sciences, University of Anbar, Ramadi, Iraq

Email

-

City

-

Orcid

-

First Name

Hasan

Last Name

Lahij

MiddleName

F.

Affiliation

Department of Medical Laboratories Techniques, College of Health and Medical Techniques University Of Al-Maarif , Al Anbar,31001, Iraq

Email

hassan.falah@uoa.edu.iq

City

-

Orcid

-

Volume

34

Article Issue

3

Related Issue

53640

Issue Date

2025-07-01

Receive Date

2025-03-12

Publish Date

2025-07-01

Print ISSN

1110-2179

Online ISSN

2537-0979

Link

https://ejmm.journals.ekb.eg/article_420367.html

Detail API

http://journals.ekb.eg?_action=service&article_code=420367

Order

420,367

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Polymorphism Effects of TLR2 and TLR4 genes on Different Otitis Media Bacterial Infections

Details

Type

Article

Created At

09 Apr 2025