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Distribution of Quinolone Plasmid Resistance Genes in ESBL and Carbapenemase Producing Escherichia coli Associated with Catheter Urinary Tract Infections

Article

Last updated: 09 Apr 2025

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Subjects

-

Tags

Antimicrobial agents

Abstract

Background: Among hospitalized cases, urinary catheter-associated urinary tract infections (CAUTIs) are important problems. Objective: Finding plasmid-mediated quinolone resistance (PMQR) genes in Escherichia coli (E. coli) from CAUTI cases in intensive care units that exhibit high quinolone antibiotic resistance was the aim of this investigation. Additionally, the researchers aimed to speculate as to what connection these genes have with carbapenemase and Extended-spectrum beta-lactamase resistance (ESBL) resistances. Methodology: Adult cases with CAUTIs hospitalized to ICUs at Mansoura University Hospital in Egypt were included in the retrospective cross-sectional investigation. All isolated E. coli were added in the research, and the ciprofloxacin-resistant organisms were chosen for additional research using multiplex polymerase chain reaction (PCR) to detect the qnr A, qnr B, and qnr S genes. Additionally, we used phenotypic analysis to discover ESBL in these organisms. Results: 204 (24.5%) E. coli isolates from CAUI cases were found during the study period. Using the MIC technique, fifty of these isolates showed ciprofloxacin resistance. In 26 isolates (52%), the double disc technique detected ESBL, whereas in 23 isolates (46%), CDT detected carbapenemase. Multiplex PCR revealed that qnrA was the most common gene (84%), followed by qnrB (46%), and qnrS (34%). A strong correlation (P = 0.001) was found between an increase in ciprofloxacin MIC and the presence of numerous qnr genes.The qnrA (88.5%), qnrB (50%) and qnrS (26.9%) genes are present in high ESBL E. coli. Additionally, the qnrA gene (78.2%), qnrB gene (60.9%), and qnrS gene (26.7%) are present in many E. coli carbapenemase producers. Conclusions: The study examines the frequency of QR genes mediated by ciprofloxacin-resistant plasmids in E. coli derived from hospital-acquired CAUTI. The qnr genes were often linked to carbapenem resistance, ESBL, and other forms of antibiotic resistance.

DOI

10.21608/ejmm.2025.371300.1533

Keywords

Escherichia coli, Multiplex Polymerase Chain Reaction, Plasmid-Mediated Quinolone Resistance, Catheter-associated Urinary Tract Infections, Extended-Spectrum Beta-Lactamase Resistance

Authors

First Name

Mohamed

Last Name

Ismaeil

MiddleName

S.

Affiliation

Clinical Pathology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt 35511

Email

salehmansoura2024@gmail.com

City

-

Orcid

-

First Name

Ahmed

Last Name

Elewa

MiddleName

M.

Affiliation

Clinical Pathology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt 35511

Email

elewaah@hotmail.com

City

-

Orcid

-

First Name

Nermin

Last Name

Abo El Kheir,

MiddleName

Y.

Affiliation

Clinical Pathology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt 35511

Email

nyboelk@hotmail.com

City

-

Orcid

-

First Name

Maysaa

Last Name

Zaki

MiddleName

E.

Affiliation

Clinical Pathology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt 35511

Email

may_s@mans.edu.eg

City

-

Orcid

-

First Name

Ehab

Last Name

Fahmy

MiddleName

M.

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Helwan University, Helwan 11731, Egypt

Email

ehabfahmy@gmail.com

City

-

Orcid

-

First Name

Karim

Last Name

Montasser

MiddleName

A.

Affiliation

Clinical Pathology Department, Faculty of Medicine, Helwan University, Helwan 11731, Egypt

Email

kareem@hotmail.com

City

-

Orcid

-

Volume

34

Article Issue

3

Related Issue

53640

Issue Date

2025-07-01

Receive Date

2025-03-25

Publish Date

2025-07-01

Print ISSN

1110-2179

Online ISSN

2537-0979

Link

https://ejmm.journals.ekb.eg/article_420300.html

Detail API

http://journals.ekb.eg?_action=service&article_code=420300

Order

420,300

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Distribution of Quinolone Plasmid Resistance Genes in ESBL and Carbapenemase Producing Escherichia coli Associated with Catheter Urinary Tract Infections

Details

Type

Article

Created At

09 Apr 2025