This research aims to study the factors affecting the environmental guidance awareness of farmers in Assiut Governorate in the field of safe use of agricultural pesticides. A random sample of 350 farmers was selected from the villages of the governorate that were geographically divided, then an administrative center was selected from each geographical region, then a village was randomly selected from each center. The data was collected using a questionnaire form through a personal interview, then the data was collected, reviewed and analyzed using the Chi-square, Tcher and Kramer tests. Frequencies and percentages were also used in the description. It became clear from the data analysis that age, educational level, size of agricultural holdings, degree of social participation, cultural openness, and number of information sources, significantly affect the dependent variable, which is the level of environmental guidance awareness by 23%, and that the most influential of them is the information sources. The most important problems facing the sample members when using pesticides are not using them in the correct way and high prices, as well as methods of disposing of empty containers to preserve the environment. Therefore, the research recommends holding many seminars in the guidance centers in the villages in this field, and conducting field experiments in this field, with the help of satellite channels and social media to help spread their recommendations and know the needs of their use.