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419972

New Horizons for the Relationship between Genetic Variations and Two Immune Markers in Spontaneous Abortion among Iraqi Women: A Cross-Sectional Study

Article

Last updated: 09 Apr 2025

OverviewSimilar Items

Subjects

-

Tags

Clinical microbiology

Abstract

Background: Spontaneous abortion (SA) is a significant health concern in Iraq. It can be caused by genetic, immunological, and environmental factors. Objective: The aim of this study is to identify the association between CD34+ and HLA-F levels with Toll-like receptors (TLR2, TLR4, TLR7) polymorphisms and their predisposing to SA. Methodology: A survey involving 200 participants was conducted, comprising 100 women diagnosed with SA at the beginning of pregnancy and 100 pregnant women serving as a control group of the same gestational age, all were referred to two Maternity and Children Hospitals in Diyala and Babylon, Iraq. The age range of participants was 20 to 40 years. ELISA was used to quantify the concentrations of immune parameters CD34+ and HLA-F. PCR-RFLP technique was done to evaluate variations in three Toll-like receptor genes. Results: A statistically significant association (p-value ≤0.001) between CD34+ and HLA-F concentrations and a risk factor for SA. The TLR4 (rs1927914) (G/A) genetic variation has revealed a novel association between the genotype GG (2.97±0.7ng/ml) and SA, which have higher levels of HLA-F concentration in peripheral blood of aborted women at a significant value (P-value ≤0.006) compared with other genotypes. Conclusions: The research showed lower CD34+ and HLA-F concentrations in SA women and a novel association between Toll-like receptor 4 genotype GG and elevated HLA-F concentration in Iraqi women experiencing spontaneous abortion and needs further investigations.

DOI

10.21608/ejmm.2025.368257.1520

Keywords

ELISA Method, RCR-RFLP Technique, Miscarriage, Single nucleotide polymorphism

Authors

First Name

Nedhal

Last Name

Kaleefah

MiddleName

M.

Affiliation

Department of Microbiology, College of Medicine, Babylon University, 32001, Iraq

Email

nedalalazzwy@gmail.com

City

-

Orcid

0000-0002-3267-6238

First Name

Bushra

Last Name

Al-Tmimi

MiddleName

J.

Affiliation

Department of Microbiology, College of Medicine, University of Babylon, 51001, Iraq

Email

jbushra468@gmail.com

City

-

Orcid

-

First Name

Asmaa

Last Name

Gataa

MiddleName

K.

Affiliation

Department of Obstetrics and Gynecology, College of Medicine, University of Babylon, 51001, Iraq

Email

asmakadhim@gmail.com

City

-

Orcid

0000-0003-1209-1379

Volume

34

Article Issue

2

Related Issue

51523

Issue Date

2025-04-01

Receive Date

2025-03-15

Publish Date

2025-04-01

Page Start

425

Page End

433

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 34_Issue 2_Pages 425-433.pdf

PDF . 888.2kB

Link

https://ejmm.journals.ekb.eg/article_419972.html

Detail API

http://journals.ekb.eg?_action=service&article_code=419972

Order

419,972

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

New Horizons for the Relationship between Genetic Variations and Two Immune Markers in Spontaneous Abortion among Iraqi Women: A Cross-Sectional Study

Details

Type

Article

Created At

09 Apr 2025