ManageChatDiscover
419970

Coliphage ASEC2202-Driven Eradication of Biofilm Matrix of E. coli Clinical Isolate

Article

Last updated: 09 Apr 2025

OverviewSimilar Items

Subjects

-

Tags

Antimicrobial agents

Abstract

Background: Biofilm-producing multidrug-resistant (MDR) Escherichia coli remains a prominent global health concern and pose a formidable challenge to conventional antimicrobial therapies. Bacteriophage therapy has gained substantial attention as they target bacterial cells, often without disturbing the commensal microbiota, and can penetrate biofilm matrices, making them promising candidates for biofilm eradication. Objective: In this study, we evaluated anti-biofilm efficacy of ASEC2202, a novel lytic coliphage, isolated from sewage water in our lab. Methodology: The biofilms were produced using eight distinct E. coli MDR strains obtained from clinical specimens. The anti-biofilm activity of ASEC2202 bacteriophage was analysed using TCP method. Results: ASEC2202 exhibited a latent period of 30 minutes and a burst size of approximately 552 plaque-forming units (PFU) per infected cell. The phage demonstrated significant bactericidal activity, reducing E. coli population by approximately 3 logarithmic units (∼99.9% reduction) within 10 hours of incubation. Coliphage ASEC2202 exhibited potent lytic activity against biofilm-producing MDR E. coli, with biofilm reduction varying by stage and phage concentration. In early-stage biofilms, reductions ranged from 68.66% to 66.52%, while mid-stage biofilms showed over 75% reduction at 54 hours post-infection. For mature biofilms, titrations of 10⁵ and 10⁷ PFU/mL achieved reductions of 70.16% and 69.75%, respectively. Conclusion: The study concludes that while mature biofilms exhibit increased resilience, targeted phage therapy with optimized concentrations remains effective for biofilm mitigation. However, further in vivo studies are necessary to fully explore therapeutic potential of coliphage ASEC2202.

DOI

10.21608/ejmm.2025.362266.1490

Keywords

bacteriophage, Antimicrobial resistance, Biofilm, MDR, mitigation

Authors

First Name

Sudhakar

Last Name

Padmesh

MiddleName

-

Affiliation

1Amity Institute of Biotechnology, Amity University Uttar Pradesh, Lucknow Campus, Gomti Nagar Extension, Lucknow - 226028 (INDIA)

Email

sudhakarparadox@gmail.com

City

-

Orcid

-

First Name

Humaira

Last Name

Saeed

MiddleName

-

Affiliation

1Amity Institute of Biotechnology, Amity University Uttar Pradesh, Lucknow Campus, Gomti Nagar Extension, Lucknow - 226028 (INDIA)

Email

humasaeid@gmail.com

City

Lucknow

Orcid

-

First Name

Aditi

Last Name

Singh

MiddleName

-

Affiliation

1Amity Institute of Biotechnology, Amity University Uttar Pradesh, Lucknow Campus, Gomti Nagar Extension, Lucknow - 226028 (INDIA)

Email

singh.aditi00@gmail.com

City

-

Orcid

0000-0002-9303-5960

First Name

Manodeep

Last Name

Sen

MiddleName

-

Affiliation

Department of Microbiology, Dr. Ram Manohar Lohia Institute of Medical Sciences, Lucknow (INDIA)

Email

sen_manodeep6@yahoo.com

City

Lucknow

Orcid

-

Volume

34

Article Issue

2

Related Issue

51523

Issue Date

2025-04-01

Receive Date

2025-02-20

Publish Date

2025-04-01

Page Start

415

Page End

424

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 34_Issue 2_Pages 415-424.pdf

PDF . 881.4kB

Link

https://ejmm.journals.ekb.eg/article_419970.html

Detail API

http://journals.ekb.eg?_action=service&article_code=419970

Order

419,970

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Coliphage ASEC2202-Driven Eradication of Biofilm Matrix of E. coli Clinical Isolate

Details

Type

Article

Created At

09 Apr 2025