Medical diagnosis usually requires blood analysis of various biomarkers essential for disease detection and health status monitoring. Human serum albumin (HSA) is the most abundant serum protein and is an indicator of liver and kidney diseases. Cardiac troponin 1 (cTn1) is a protein member of the cardiac troponin complex used to diagnose several pathologies associated with cardiomyocyte necrosis. Laser-induced fluorescence (LIF) is a spontaneous emission of atoms or molecules that have been excited by laser radiation. LIF is a technique with high sensitivity and specificity, and it is one of the most significant developments used as an analytical tool for qualitative and quantitative analysis. This article introduces the potential application of femtosecond LIF in clinical analysis as a new detection method for rapid monitoring of certain serum biomarkers such as HSA and cTn1. The femtosecond LIF provides a new, very sensitive, precise, and direct method for monitoring the HSA and cTn1.