Background: Infection control training sessions for recently employed nurses have an important, value especially in recent years during the outbreaks of emerging and re-emerging infections to reduce their risk to occupational infectious diseases and improve their attention to implementation of infection control policies, procedures and practices. Aim: The present study aimed to; assess the effect of infection control training sessions on recently employed nurses' knowledge and performance. Subject and Methods: A quasi-experimental design was used to conduct the study in Belqas General Hospital, Dekirnis Central Hospital, Aga Central Hospital, and Nabarro Central Hospital, at Eldakahlia Governorate. The study subjects consisted of all newly employed nurses (68 nurses). Four tools used for data collection consisted of; nurses' demographic and occupational characteristics, nurses' knowledge about infection control, nurses' performance toward infection control measures, and Nurses' feedback related to training sessions. Results: the post-test scores of nurses' knowledge and performance after implementation of the training sessions significantly improved in the studied recently nurses 101.50 ± 12.77, 134.25 ± 18.35 compared with pre-test scores with 40.41± 12.20, 65.0 ± 15.56 (P<0.001). As determined by Cohen's d test, the effect size of an educational program is quite large on the recently employed nurses' knowledge and performance. Conclusion: The training sessions about infection control was significantly improved knowledge and performance of the studied recently employed nurses. Recommendations: Conducting ongoing education to nurses to perform in adherence to the infection control standard procedures. Also, providing orientation programs for recently employed nurses about standard precautions infection control