Banana bunchy top virus (BBTV) is considered as a destructive viral disease affecting banana plants in Egypt. Recently, nanotechnology approach was used to generate resistance against plant viruses. The main objective of this study was to evaluate the antiviral capabilities of chitosan nanoparticles to control BBTV in banana plants in vitro cultured. The naturally infected banana plants were collected from open fields at different locations in Egypt. Polymerase chain reaction (PCR) was successfully amplified approximately of 747 bp using specific primers for coat protein gene.  In-vitro, three different concentrations of Chi-NPs (50, 100, and 200 mg/l) were evaluated. The results showed that infected explants with BBTV were symptomless, the enzyme-linked immunosorbent assay (ELISA) produced negative results. Biochemical analysis in infected explants showed a significant increase in polyphenol oxidase and peroxidase enzymes as well as a significant increase in total oxidant activity in compression with healthy ones. Infected explants treated with Chi-NPs showed a significant increase in total phenols, total soluble sugar, protein, and malondialdehyde, compared to those of untreated infected control. Collectively, we can conclude that Chi-NPs are a potential antiviral remedy against BBTV and could be considered in virus management.