The present experiment was performed throughout two successive seasons of 2020/2021 and 2021/2022 to intended to response of kapok (Ceiba pentandra L.) seedlings to irrigated with secondary treated sewage water (TSW), agricultural drainage water (ADW) and Nile irrigation water (NIW) either direct (100%) or mixed TSW or ADW with NIW (25, 50, and 75%) in addition to using foliar glycine betaine (GB) application at (0.0 mM and 50 mM) on vegetative growth parameters, Anatomical structures of leaf and antioxidant activity. Results showed that irrigation with directly (100%) of TSW and ADW decreased plant height, number of leaves on plant, stem diameter/plant, fresh weight of stem, leaves and roots, root diameter, dry weight of stem, leaves and roots, leaf area/ plant, root length, leaf thickness, leaf mid-vein; however, increased free proline content, total phenolic content and antioxidant activity. As well as, GB significantly increased all vegetative growth parameters, free proline content, total phenolic content, leaf thickness, leaf mid-vein and antioxidant activity. But also with irrigation kapok seedlings at TSW or ADW and NIW at 25 and 50% give better results likewise irrigation with NIW for all parameters. Combination with GB and water treatments give better results at 50 mM GB and TSW or ADW at 25 and 50% mixing with NIW. Concluded that from irrigation kapok seedlings with TSW or ADW preferably was mixed with NIW percentage 50:50 gave better results on vegetative growth characters of kapok leaves seedlings like control seedlings irrigated with NIW, and spraying of GB principally at the concentration of 50 mM combined together has the possibility enhancing and progressing the characters of kapok.