This study aimed to explore the impacts of SPIDES every 4 days intervals for 0, 45, 90, and 135 min on internal characteristics, and early embryonic development of eggs stored for various periods (1, 2, 3, 4, and 5 weeks). Total of 7500 eggs, weighing 62 g, were split into five groups at random, each containing 1350 eggs and three duplicates of 450 eggs; 150 eggs were included in each treatment group as a control negative (non-SPIDES). Results indicated the highest dynamic weight loss (DWL) in non-SPIDES eggs at all storage periods. SPIDES decreased DWL on Days 4, 8, 12, 16, and 20 days of storage. The lowest DWL was observed on Day 4 for eggs stored for 2wk and SPIDES for 90 min. Values of pH for pre- and post-SPIDES of albumen and post-SPIDES of yolk were not affected by storage period, SPIDES protocol, and their interaction. Pre-SPIDES, yolk pH values on Day 4 decreased (P<0.05) in eggs stored more than 4wk as compared to 1wk storage period, being the lowest in eggs stored for 1 and 4wk with SPIDES for 135 min, eggs stored for 2 and 3 with SPIDES for 45 min, and eggs stored for 5wk with SPIDES for 90 min. Blastoderm diameter was higher (P<0.05) in eggs stored for 4wk than other storage periods, and eggs with SPIDES for 135 min than other minuets on all Incubation hours (12, 18, and 24h). In eggs stored for all periods. Blastoderm diameter was the highest in eggs with SPIDES for 135 min and the lowest in non-SPIDES eggs at all Incubation hours.
 In conclusion: Short Periods of Incubation During Egg Storage (SPIDES) every 4 days during storage period has been exhibited improvements in dynamic weight loss, maintained pH values of albumin and yolk, and increased diameter of blastoderm of embryos at early embryonic developmental stage for eggs stored for one week or more.