The Suez Gulf is one of the richest fishing grounds of Red Sea in the Egyptian sector, it receives pollution from different sources. The main objective of this study is to use some fish species as bioindicator to environmental pollution. Three fish species were sampled from 3 different sites; Site 1 (Clean Area), site 2 (Petroleum & fishing activities) and site 3 (Al-Attaka commercial landing site). The Collected fish samples were: A) Dussumieria spp., B) Scomberomorus Spp. and C) Sphyraena Spp. The detection of elevated concentrations of total and fecal coliforms suggests the presence of microbial contamination. The results of oxidative stress displayed significant (P<0.01) increase in the level of ROS formation (259.0±10.4) in site-3 in comparison to site-1 (136.0±5.5) (control location). Results of DNA adducts revealed that site-3 showed significant (P<0.01) increase in the levels of 8-OHdG (18.37±1.47) compared with those in site-1 (5.75±0.77). Apoptosis results demonstrated significant (P<0.01) increase in the levels of apoptosis (32.79±2.92) in site-3 in comparison to site-1 (11.52±1.46) considered as control site. However, results of DNA barcoding did not reveal changes in the DNA sequence of the three fish strains. So, the present study suggested that oxidative stress resulted from environmental pollution could influence the stress biomarkers, however it, could not induce nucleotide replacements in fish strain under study.