Gill nets are widely used in small-scale fisheries because of their simplicity and efficiency, particularly for capturing demersal and pelagic species. This study analyzed the catch characteristics of bottom-set gill nets with a 5.08cm mesh size during the evening (7:00–8:30 PM) and pre-dawn (4:00–5:30 AM) periods in Inner Ambon Bay, Indonesia. A total of 30 fishing trips were conducted, and data on species composition, catch diversity, and length frequency distribution were collected. The results revealed significant temporal variation in species composition and dominance. In the evening, Selar crumenophthalmus was the most dominant species (64.57%), whereas its proportion decreased to 38.15% in the pre-dawn period, accompanied by an increase in Upeneus moluccensis (19.61%). Diversity indices (Shannon-Wiener) indicated moderate diversity, with higher values during the pre-dawn period (1.51) than during the evening (1.36). Dominance was lower in the pre-dawn period (0.09) than in the evening (0.44). Length frequency analysis showed that the gilled method was the most effective capture mechanism, selectively targeting fish in the 21.5–22.5cm range. These findings emphasize the influence of fishing time on catch composition and size selectivity, providing insights for optimizing fishing practices and supporting sustainable fishery management in Inner Ambon Bay.