An experiment was conducted to assess the culture and production of spirulina (Spirulina platensis) in three distinct concentrations of digested rotten banana supernatants (Musa acuminata), and Kosaric medium (KM) as control. Two hundred grams of rotten banana was allowed to digest using aeration in 5.0L glass jar. After 30 days, an almost colorless supernatant was obtained. This supernatant was filtered using a 30 µm mesh and supplemented with 9.0 g/L NaHCO₃, 0.20 g/L urea, and micronutrients. Three distinct concentrations of the supernatant (20, 40, and 60%) were prepared, each with three replications. Spirulina reached its maximum cell weight (dry wt. basis) of 16.44 ± 0.78mg/ L in KM followed by 11.876 ± 0.798, 9.724 ± 0.288 and 1.833 ± 0.263mg/ L in the supernatants of 40, 60 and 20% DRBM, respectively, on the 10th day of culture. A comparable pattern was also noted in the case of visual density of the media contained spirulina, total biomass (mg/L), chlorophyll a content (mg/L), specific growth rates based on cell weight and chlorophyll a) of spirulina. Cell weight of S. platensis had significant (P<0.05) direct correlation with chlorophyll a (r = 0.426) of spirulina grown in the supernatants of different DRBM, and Kosaric medium. On the other hand, total biomass of S. platensis had a significantly (P< 0.05) close relationship with chlorophyll a (r= 0.404) of spirulina grown in different DRBM supernatants, and Kosaric medium. The results showed a substantial (P<0.05) and direct correlation between the cell weight and the total biomass of spirulina (r = 0.421) of spirulina grown in the supernatant of different DRBM, and Kosaric medium. We might conclude that 40% digested rotten banana medium can be used for S. platensis mass cultivation.