The hybrid red tilapia is highly sought after worldwide due to its delicious flavor, attractive appearance, ease of cultivation, adaptability to challenging environments, strong disease resistance, short generation time, and rapid growth. In the red tilapia hatchery, there is difficulty spawning of some red strains of tilapia and low viability red tilapia eggs and fry. Therefore, this study aimed to evaluate the function of different levels of vitamin E on reproductive performance and histological sections of hybrid female red tilapia Oreochromis spp. postspawning (at 60^th days). Females were divided into four groups, including 4 vitamin E treatments (0, 50, 100, and 200mg kg^-1 diet). Each group had 3 replicates. The results showed that Vit. E had an effective impact on reproductive performance as oocyte diameter, egg diameter, fecundity, hatching rate, spawning percentage, and larval length at 60^th day post-spawning and on histological sections of ovaries that showed high maturity with revealed yolk granules and yolk plates. In conclusion, adding vitamin E with different doses (50, 100, and 200mg/ kg of feed) had an effective influence on the reproductive performance and histological sections of the ovaries of females of hybrid red tilapia. Supplementing with a 50mg/ kg Vit. E dosage showed to be sufficient for a high reproductive performance of females of the hybrid red tilapia hatchery.