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IN VITRO: INHIBITION OF PARTIALLY PURIFIED PANCREATIC OVINE LIPASE BY WILLOW BARK EXTRACTS

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Last updated: 09 Apr 2025

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Abstract

Obesity has existed for a very long time. Obesity leads to improper physiological metabolism, which in turn produces a host of physiological and social issues in addition to aesthetic concerns. Individuals are working to discover anti-obesity medications and other safe and efficient treatment options. Because pancreatic lipase (PL) is essential to human fat metabolism, PL inhibitors are now used to treat obesity in clinical settings. This research involved partially purifying lipase from the ovine pancreas. One lipase isoenzyme was identified using the CM-Cellulose ion exchange chromatography technology. It had a specific activity of 2.467 U/mg protein and a recovery of 272 in comparison to the crude extract. The molecular weight was determined to be 33.8 kilodaltons, and the electrophoresis method produced only one band. Using p-nitrophenyl acetate as a substrate, the capacity of willow bark extracts to inhibit partly purified pancreatic lipase activity was investigated.
The inhibitory percentages via treating the enzyme with EthOH 70% and H2O were found to be 27.14 and 61.43 respectively. Furthermore, the Lineweaver-Burk plots revealed the inhibition modes noncompetitive with both extracts.

DOI

10.21608/jbaar.2025.339535.1113

Keywords

Ovine Pancreatic Lipase, Willow bark, extracts, Purification, inhibition

Authors

First Name

Fatima Murhaf

Last Name

Alrushdi

MiddleName

Mohammed

Affiliation

College of Education for Pure Science, University of Mosul, Department of Chemistry.Mosul, Iraq.

Email

fatima.murhaf@gmail.com

City

-

Orcid

-

First Name

Omar Younis

Last Name

Mohammed Al-Abaasy

MiddleName

-

Affiliation

3 Department of Chemistry, College of Education for Pure Science, University of Mosul, Mosul, Iraq.

Email

omar.younis@yahoo.com

City

-

Orcid

-

First Name

Ruaa Nashat

Last Name

Al-Saffar

MiddleName

-

Affiliation

Anesthesia Techniques Department, College of Health and Medical Techniques, Al-Mustaqbal University, 51001, Babylon, Iraq

Email

ruaa.al-saffar@gmail.com

City

-

Orcid

-

First Name

Hadi

Last Name

Abbood

MiddleName

Yasir

Affiliation

Medical Laboratories Techniques Department, College of Health and Medical Techniques, Al-Mustaqbal University, 51001, Babylon, Iraq.

Email

hadi.abbood70@gmail.com

City

-

Orcid

-

First Name

Ahmed

Last Name

Al-Hamairy

MiddleName

Khudhair

Affiliation

Anesthesia Techniques Department, College of Health and Medical Techniques, Al-Mustaqbal University, 51001, Babylon, Iraq.

Email

ahmed.khudhair@gmail.com

City

-

Orcid

-

First Name

Mohanad

Last Name

Saleh

MiddleName

Yakdhan

Affiliation

Department of Chemistry, College of Education for Pure Science, University of Mosul, Mosul, Iraq.

Email

mohanad.yakdhan.saleh@gamil.com

City

-

Orcid

-

First Name

Heba

Last Name

Abdelzaher

MiddleName

-

Affiliation

Department of Clinical pharmacy, Faculty of Pharmacy, Minia University, 61519 Minia, Egypt.

Email

hebaseoud88@gmail.com

City

-

Orcid

-

First Name

Mohamed

Last Name

Abdelzaher

MiddleName

Abuelseoud

Affiliation

Environmental Science and Industrial Development Department, Faculty of Postgraduate Studies for Advanced Sciences, Beni-Suef University, Beni-Suef 62511, Egypt.

Email

m.abuelseoud@psas.bsu.edu.eg

City

Beni-Suef

Orcid

0000-0001-5180-7283

First Name

Mahmoud

Last Name

A. Kenawy

MiddleName

-

Affiliation

Biophysics Branch, Department of Physics, Faculty of Science, Al Azhar University, Nasr City 11884, Cairo, Egypt./ Radiology Techniques Department, College of Health and Medical Techniques, Al-Mustaqbal University, 51001, Babylon, Iraq

Email

mahmoud.a.kenawy80@yyahoo.com

City

-

Orcid

-

Volume

11

Article Issue

1

Related Issue

53815

Issue Date

2025-03-01

Receive Date

2024-11-26

Publish Date

2025-03-01

Page Start

168

Page End

179

Print ISSN

2356-9174

Online ISSN

2356-9182

Article File

JBAAR_Volume 11_Issue 1_Pages 168-179.pdf

PDF . 1.2MB

Link

https://jbaar.journals.ekb.eg/article_402670.html

Detail API

http://journals.ekb.eg?_action=service&article_code=402670

Order

402,670

Type

Original Article

Type Code

1,272

Publication Type

Journal

Publication Title

Journal of Bioscience and Applied Research

Publication Link

https://jbaar.journals.ekb.eg/

MainTitle

IN VITRO: INHIBITION OF PARTIALLY PURIFIED PANCREATIC OVINE LIPASE BY WILLOW BARK EXTRACTS

Details

Type

Article

Created At

29 Mar 2025