Half of the world's population has Helicobacter pylori diseases. This study evaluated Iraqi controls and patients to see if rs2981572 interleukin 20 gene polymorphism promotes renal disease. Half of 300 individuals were patients and half were controls in this study. Each participant supplied 8 ml of venous blood, however only 4 ml was immediately placed in EDTA tubes to capture DNA. The two trial groups used a gel tube to coagulate and separate serum for IL-20 measurements. Forward and reverse primers were used to amplify the IL-20 gene and rs2981572 polymorphism using tetra primer amplification refractory mutation The ELISA test measured IL-20. The Results were shown In relation to rs2981572, the TT genotype was protective (OR = 0.54, 95% CI = 0.34–0.85, P = 0.025) and the TG genotype risky. GG genotype did not increase disease risk (OR = 1.2, 95% CI = 0.50–3.10, P = 0.58). The T and G alleles had substantial protective factors (OR = 0.67, 95% CI = 0.48–0.93) and risk factors (OR=1.49, 95%CI =1.07–2.08) (P = 0.023). We identified a strong link between H. pylori-related urological illnesses and rs2981572 polymorphism genotypes and allele frequencies. Both studies estimated IL-20 from whole blood serum. IL-20 levels were significantly higher in cases compared to controls (1348.24 ± 29.09 vs. 812.91 ± 29.63 pg/ml, P = 4.7×10⁻²⁰). IL-20 was high in 63% of people. The TG genotype and G allele in the rs2981572 polymorphism may be linked to H. pylori infections in renal disease patients.