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417885

Molecular Characterization of Multidrug-Resistant Enterobacter spp. Isolated from Powdered Infant Formula and children with Gastroenteritis

Article

Last updated: 29 Mar 2025

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Subjects

-

Tags

Antimicrobial agents

Abstract

Background: Enterobacter species causes many serious and life-threatening infections. Enterobacter species may be the cause of diarrhea in children. Multidrug-resistant (MDR) Enterobacter species limits treatment options. Powdered Infant Formula (PIF) was epidemiologically linked to diseases in infants caused by Enterobacter spp. Objectives: Our study aimed to detect the frequency and antimicrobial resistance profile, both phenotypically and genotypically of MDR Enterobacter isolated from powdered infant formula (PIF) and stools of children suffering from gastroenteritis who needed hospitalization and determine if there is a link between the presence of Enterobacter spp. in contaminated PIF & its presence in the stool of infants. Methodology: Isolation of Enterobacter spp. from Powdered Infant Formula and Stool of Infants, Antimicrobial susceptibility test of Enterobacter spp. isolates to antimicrobial agents, genotypic test by polymerase chain reaction (PCR) for five genes: 16S rDNA, blaSHV, blaTEM, aac(6′)-Ib-cr, and qnrB1 genes, and molecular identification of multidrug-resistant of Enterobacter strains which will be isolated from infant formula and stools. Results: Frequency of multidrug-resistant Enterobacter spp. isolates from PIF were 3 isolates positive for four resistant genes in three antibiotics classes (β-Lactam, Aminoglycoside, and Fluoroquinolones) with a percentage of 27.3%, and the genotypic frequency of multidrug-resistant Enterobacter spp. isolates from infants' stool were 2 isolates positive for four resistant genes in three antibiotics classes (β-Lactam, Aminoglycosides, and Fluoroquinolones) with a percentage of 13.3%. Conclusion: MDR Enterobacter was isolated from young aged (less than 24 months) children and PIF, more than one resistance gene: blaSHV, blaTEM, aac(6′)-Ib-cr, and qnrB1 genes were detected in isolates. The presence of MDR strains is risky at a young age as it limits treatment options. Drug-resistant genes may be transmitted to a child through a carrier mother or PIF or cross-infection from the hospital. 

DOI

10.21608/ejmm.2025.366063.1511

Keywords

Enterobacter spp, Antimicrobial resistance, Multidrug-resistance

Authors

First Name

Armia

Last Name

Habib

MiddleName

O.

Affiliation

Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt

Email

armiaonsy2@gmail.com

City

Sohag

Orcid

-

First Name

Ismael

Last Name

Mohamed

MiddleName

S.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt

Email

ismailsoliman37@aun.edu.eg

City

Assiut

Orcid

-

First Name

Shereen

Last Name

Galal

MiddleName

M.

Affiliation

Department of Pediatrics, Assiut University Children Hospital, Faculty of Medicine, Assiut University, Assiut, Egypt

Email

shereen80@aun.edu.eg

City

Assiut

Orcid

-

First Name

Rawhia

Last Name

Abd El Hamid

MiddleName

F.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt

Email

rawhiafathy@aun.edu.eg

City

Assiut

Orcid

-

Volume

34

Article Issue

3

Related Issue

53640

Issue Date

2025-07-01

Receive Date

2025-03-06

Publish Date

2025-07-01

Print ISSN

1110-2179

Online ISSN

2537-0979

Link

https://ejmm.journals.ekb.eg/article_417885.html

Detail API

http://journals.ekb.eg?_action=service&article_code=417885

Order

417,885

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Molecular Characterization of Multidrug-Resistant Enterobacter spp. Isolated from Powdered Infant Formula and children with Gastroenteritis

Details

Type

Article

Created At

29 Mar 2025