Grape, Vitis vinifera L. is considered the most economically important fruit crops in the world. In Egypt, grape comprise the second after citrus for domestic consumption. This study was conducted in the Experimental Horticultural Farm of South Valley University, Qena, to conduct a survey on the most important predominant genera of plant parasitic nematodes (PPNs), associated with the soil rhizosphere and roots of grape trees, Vitis vinifera L. cv. Superior Seedless, that causing remarkable economic losses. The obtained results reported that nine genera of PPNs were associated with soil rhizosphere of grape trees namely, Meloidogyne, Tylenchorhynchus, Longidorus, Pratylenchus, Xiphinema, Trichodorus, Tylenchulus, Helicotylenchus and Paratylenchus. While, only three genera were reported to be in grape root samples viz., Meloidogyne, Pratylenchus, and Tylenchulus. The results revealed that the population density (PD) of Meloidogyne spp. was the most dominant, attacking grape trees with an average of 2550.78 individuals per 200 cm³ soil, and 1228.89 individuals/1g roots, with 37.99% and 55.36 % frequency of occurrence (FO) in soil and root samples, respectively. Statistical analysis of soil and root results reported that there were significant differences in nematode populations among registered genera LSD 5% = 154.8 and 292.4, respectively. Additionally, the numbers of nematode populations in soil and root samples varied significantly between February, March, and January, with the LSD 5% values being 369.3 and 338.4, respectively.