Background: Biomedical Waste Product Management (BMWM) is a critical component of healthcare because improper management possess significant risks to public health and environment. Aim: to assess staff nurses' knowledge and attitudes regarding biomedical waste management. Research design: A descriptive correlational design was utilized in this study. Setting: The study was conducted at Cardiothoracic Minia University Hospital. Subjects: All available staff nurses (N=256), employed at the hospital during the data collection period participated in the study. Tools: two instruments were used: tool (I) A questionnaire comprising personal data, and the Bio-Medical Waste management Structure Questionnaire. Tool (II) Bio-Medical Waste Structured Knowledge Questionnaire. Results: More than half (59.8%) of staff nurses had poor knowledge and a negative attitude toward biomedical waste management. Conclusion: A highly statistically significant correlation was observed between staff nurses' overall knowledge and their attitude regarding toward BMWM at Cardiothoracic Minia University Hospital. Recommendation: Its recommended to enhancing in-service training programs, enforcing compliance, with waste management protocols, allocating necessary resources, conducting periodic assessments, and promote awareness of to ensure safer healthcare and environmental practices.