Feline hyperesthesia syndrome (FHS) is a little-known syndrome characterized by skin texture fluctuations and behavioral disturbances (self-mutilation, excessive vocalization, jumping, running, tail chasing, etc.). Several long-term attacks of neurodermatological findings were evident in 23 cats of various breeds and both sexes. Depending on the respective clinical response, fecal microbiota transplantation (FMT) was used with heterologous origin between 1 and 4 sessions. Considering the frequency of attacks for clinical signs before FMT intervention, the vast majority of cats exhibited daily attacks/excessive grooming of the flank, lumbar or perineal region, lumbar hyperesthesia, vocalization, mutilation, alopecia, and allodynia. However, the disease phenotypes for FHS and the types of accompanying behavioral disorders recorded before and after FMT suggested that FMT was effective. Three different stress factors and environmental conditions were evaluated separately, showing that loud vocalization, struggling, panting, tremors and hyperesthesia decreased dramatically in response to FMT. In general interpretation, FMT was highly effective and no side effects were noticed or detected. The overall clinical response rate after FMT was 86.95%. Among them, 16 (69.56%) cats showed clinical remission, 4 (17.39%) cats showed clinical improvement and three (13.04%) cats had invalid clinical symptoms.