This study aimed to explore the effects of short periods of incubation during egg storage (SPIDES) at 4-d intervals for 0, 45, 90, and 135 min on hatchability and chick quality during varied storage durations (1, 2, 3, 4, and 5 weeks). 7500 eggs (62g each) were randomly divied into five groups,each containing 1350 eggs and three duplicates of 450 eggs; 150 eggs were included in each treatment group as a control negative (non-SPIDES). Results reflected that the hatching % decreased when storage incubation eggs over one week. Because the chick quality of the hatching chicks declined with the length of the hatching egg storage period, the hatching results were correlated with the quality of the resulting chicks. The current study's findings indicate that the SPIDES protocol Significantly enhanced hatchability stored for up to five weeks and even for just one week. The results also showed That chick quality improved significantly with SPIDES. In conclusion: Short Periods of Incubation During Egg Storage (SPIDES) every 4 days during storage period has been improved hatchability rate percent, chick quality, and early embryonic mortality stage Ι (0-3 days) for eggs stored for three weeks or more.