Background: Bioceramic sealers have been developed for 30 years to be used in
endodontics, they are ceramic materials that include zirconia, alumina, glass ceramics,
bioactive glass, calcium phosphate and hydroxyapatite with superior biological and
physical properties. Aim: To determine the effect of CeraSeal and Bio-C Sealer on
penetration depth in a comparison with AH Plus using confocal laser scanning electron
microscope (CLSM). Material and methods: This research was approved by the ethics
committee of Suez Canal University-Egypt (No.256 /2020), thirty-three single rooted
human premolars were extracted, collected and divided randomly into three groups
according to sealer type AH Plus, Bio-C Sealer and CeraSeal. Each group (n=11),
before obturation, Rhodamine B dye was mixed with each sealer, then obturation done
using single cone technique, after that the samples were cut in cross-sectional direction
at (3, 6 and 9 mm) to determine the depth of penetration using CLSM. Results: For the
maximum depth of penetration, Bio-C Sealer showed the maximum penetration, with
no statistically significant difference between the three sealers. While the results of
penetration %, showed that the Bio-C sealer had the best penetration percentage with
statistically significant difference between the three sealers. Conclusion: Within the
limitations of this study, Bio-C sealer showed the best penetration into dentinal tubules
between the tested sealers.