Introduction: Osteoporosis is a bone disorder with significant alterations in bone
biologic material and consequent bone structural distraction. Low-level laser therapy
(LLLT) has been studied as a physical modality that influences cellular activity through
photochemical, photophysical, and photobiological mechanisms, making it an effective
intervention for osteoporosis. Aim of the study: The current study was conducted to
evaluate the impacts of low-level laser therapy on glucocorticoid induced osteoporosis
at different time intervals. Methodology: This investigation involved 40 adult male
albino rats, which were evenly distributed into four groups, each containing ten rats;
control, DEX, 7 days LLLT, and 25 days LLLT. At the end of the experiment, all the
rats were humanely euthanized. Their lower jaws were then collected and subjected to
staining with Hematoxylin & Eosin (H&E) and immunohistochemical (IHC) analysis
for RANKL and OPG. Results: DEX group revealed massive alveolar bone destruction
with strong RANKL and weak OPG immunoreactivities. However, 7 days LLLT and
25 days LLLT groups showed improvements in the alveolar bone architecture with
decreased RANKL and increased OPG immunoreactivities. Conclusion: Through
RANKL/OPG ratio downregulation, LLLT improved bone architecture, encouraged
bone healing, and stimulated osteogenesis over time.