Aim: this study aimed to evaluate the effect of final rinse with sodium chloride (normal saline) versus Ethylenediaminetetraacetic acid (EDTA) on dentin erosion. Material & methods: twenty lower second premolars were selected and prepared Under Dental loupes with 6X magnification, the selected teeth will be cleaned of calculus and soft tissue remnants using a hand curette. The teeth were disinfected using 5.25% NaOCl for 10 minutes and was rinsed with distilled water. Then teeth were decapitated. All crowns were cut by a diamond stone to establishing a unified length of 14 mm for all roots. After shaping the selected premolars by rotary file till 40.04 by 5.25% NaOCl the final rinse was sodium chloride group A1. (Normal saline), sodium hypochlorite and Ethylenediaminetetraacetic acid (EDTA) group A2. Results & Conclusion: EDTA scored high dentin erosion in comparison with sodium chloride.