This study was conducted to identify the main causes of reproductive disorders in buffalo in some villages in Menoufia Governorate. The relationship between these disorders and changes in some blood components was also studied. A total of 77 female buffaloes with delayed postpartum pregnancy for more than 90 days were examined by rectal palpation. The concentrations of calcium, phosphorus, glucose, insulin, follicle-stimulating hormone (FSH), luteinizing hormone (LH), progesterone (P4), and estrogen (E2) were assessed in the blood serum. The findings showed that ovarian disorders were the most prevalent, occurring at a rate of 56.4%, followed by endometrial disorders (43.65%). Calcium and insulin levels were notably affected by the seasonal variations throughout the year. Calcium concentration exhibited a significant increase during the colder months, measured at 8.07 ±0.37, in contrast to the lower levels recorded during the hotter months, which stood at 7.16 ±0.34. The opposite trend was recorded for insulin without a significant difference. The examined reproductive disorders had a significant impact (P > 0.01) on calcium, insulin, and phosphorus levels, while their effect on glucose was negligible. The serum calcium concentration of buffaloes with endometriosis (8.72±0.33) was significantly higher compared to those with persistent corpus luteum (6.90±0.52) and non-significantly higher than those with smooth ovaries (7.23±0.43). Phosphorus was not significantly affected by season, while it was significantly higher in the blood serum of buffaloes with persistent corpus luteum (5.88±0.43) compared to those suffering from smooth ovary (4.90±0.35) or endometritis (4.60±0.28). The Ca:P ratio is calculated in the blood serum of buffaloes with the presented corpus luteum. During the cold months (1.57) or hot months (1.39), or those whose ovaries were smooth during the hot months (1.20), the Ca:P ratio may be less than the minimum required for effective reproduction. The blood glucose level was not significantly affected by either the season of the year or the type of reproductive disorder, the lowest value (35.33±3.70 g/dl) was recorded in buffaloes suffering from endometriosis, while the largest value (49.23±5.23 g/dldl) was recorded in those suffering from persistent corpus luteum without significant difference. FSH and LH levels were significantly affected by seasonal variations, while P4 and E2 remained unaffected. The general average of P4 concentration tended to rise during the summer months compared to what was recorded during the winter months. The studied reproductive disorders significantly affected P4 (P>