Abstract
Objectives: the objective of this study is to evaluate effect of pits and fissure sealant on streptococcus bacteria microbiologically. Subjects and methods: forty patients participated in the study. Group I (control group): ClinproTM sealant was applied to twenty primary molars of random selected patient. Group II (test group with ClinproTM sealant coated by Nano silver) ClinproTM sealant mixed with Nano silver was applied to twenty primary molars of random selected patient. Treatment protocol for both groups include Isolation of the Tooth, Acid Etching, Sealant Placement and Curing, and Sealant evaluation after placement. Microbiological assessment for quantification of the S. mutant's colonies. Results: Streptococcus count /level: At the baseline (Before), After one week, after one month, and After 3 months the difference was statistically insignificant. Streptococcus Levels percent reduction: From baseline to 1 week, the mean percent reduction of streptococcus levels was 36.2±7.2 for Group I and was 40.6±8.5 for Group II. This was statistically significant with p value = 0.087. Conclusion: Clinpro sealant alone or mixed with Nano silver was effective in reducing S. mutans biofilm formation.