This study was carried out in the 2023/2024 wheat-growing season to identify the pathogen that infected the durum wheat cultivar Beni-Sweif-5 grown in the production sector, Sids agricultural research station. The stems and spike holders of the wheat cultivar showed dark browning ring symptoms. Initial infection symptoms appeared as brown discoloration then turned into dark brownish, then followed by drooping of the spikes to the ground. This cultivar was sown in 200 feddans (83.36 hectares) grown on Sids Agricultural Research Station farm (Agric. Res. Stn.), Beni-Sweif governorate. Disease incidence ranged from 10-15 % in all the growing areas. The isolated fungus characterized morphologically as Cladosporium cladosporioides in the Mycology Dept., Plant Pathology Research Institute (PPATHRI), ARC. The internal transcribed sequence (ITS) region of isolated fungus amplified by ITS1 and ITS4 primers pair, morphological and molecular studies confirmed that the causal fungus is Cladosporium cladosporioides. Phylogenetic relationships showed high homology 100 % with other Cladosporium isolates on the Gen Bank database.This isolate is released with accession number OM722086 in the NCBI/Gene Bank database. To our knowledge, this is the first record of the fungus Cladosporium cladosporioides ondurum wheat in Egypt.