This study was carried out to investigate the tolerance to salinity stress, using three cultivated varieties. These cultivars were Diamant, Spunta and Cara. Single stem nodes were used as explant on MS medium supplemented by 0.0, 25, 50, 75 and 100 mM/L NaCl in a replicated experiment to assessment the salinity stress tolerance with respect to in vitro traits, which involved some morphological, physiochemical and microtuberisation traits. The results revealed that tests on the mean squares of genotypes were highly significant for all the studied morphological traits except for number of roots/plantlet, while it was significant for all studied physiochemical and microtuberisation traits, indicating the presence of real differences among the three cultivars under study. Both morphological and microtuberisation traits were highly influenced by levels of salinity, whereas highly significant difference was observed for these traits with no exception. The highest average of number of microtubers/plantlet was recorded for control (0.00 NaCl) with mean values 1.5, 2.0 and 1.80 for Spunta, Diamont and Cara cultivars, respectively and these values decreased with the increase NaCl concentrations. The heritability magnitude values were more than 50% for both number and weight of microtubers/plantlet, indicating that these traits are highly controlled by genotypes. From the results it could be concluded that microtubers induction ability is mainly controlled by genotypes as well as the interaction of genotypes by salinity stress levels. Also, the results recommended that in vitro selection for salinity stress is available for a suitable levels of sodium chloride ranged from25 to 100mM/L.