The current study's aim was to assess how different zinc sources affected the broilers' growth performance, serum concentrations, and economic evaluation. One-day-old "Cobb" broiler chicks (n = 192) with an average initial body weight of 44.10 g were randomly distributed into 6 groups. The 1^st, 2^nd, and 3^rd groups were supplied with inorganic zinc oxide, inorganic zinc sulfate monohydrate, and organic zinc methionine, respectively, at a level of 100 mg Zn/kg diet. The 4^th, 5^th, and 6^th groups were supplied with nanozinc oxide (NZnO) at a level of 20, 10, and 5 mg Zn/kg diet, respectively. The study exposed that NZnO at a level of 5 mg Zn/kg (G6) achieved a significant improvement (P < 0.05) in final body weight, cumulative body weight gain, feed conversion ratio, and feed efficiency. NZnO (G6) increased superoxide dismutase activity (SOD) and HDL (high-density lipoprotein) levels either significantly (P < 0.05) compared to G1, G2, G3, and G4 or numerically with G5. Adding NZnO lessens blood serum malondialdehyde (MDA), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and creatinine levels. Nanozinc oxide in G5 and G6 significantly achieved the best results in economic efficiency enhancement (P < 0.05). The nanozinc oxide groups achieved the best performance, boosted antioxidant activity, enhanced lipid profiling, and improved liver and kidney functions. The positive results were more noticeable in the G6. Therefore, applying NZnO (5 mg Zn/kg diet) is a new promising feed additive in the broiler industry.