For centuries, turmeric has been used as a potent, bioactive, and nontoxic remedy for many ailments. Low bioavailability and aqueous solubility are its only drawbacks. This study developed a turmeric nanoparticle preparation method and analyzed its behavior, average particle size, composition, dispersion, crystal shape, structure, and phase purity. AFM, SEM, XRD, FTIR, and UV-visible spectrophotometry were used to test these. Before and after turmeric nanoparticle treatment, Ica A and Ica D gene expression and antimicrobial properties were examined. Turmeric nanoparticles were prepared using green biosynthesis because they are non-toxic, environmentally friendly, and sometimes more effective against microorganisms than more expensive, energy-intensive, and potentially ecologically hazardous physical and chemical methods. FTIR peaks indicated pure nanoturmeric, but absorption peaks in this study showed that the samples' main components were "curcuminoids." Additionally, turmeric nanoparticles were spherical and averaged 49.8 nm. Decomposition analysis and antioxidant testing showed that turmeric nanoparticles do not cause hemolysis or harm humans. Turmeric nanoparticles inhibited S. aureus growth more in aqueous nanoparticles. This inhibition occurred at 1000 g/ml turmeric nanoparticles. Perform real-time PCR to determine how turmeric nanoparticles affect Staphylococcus aureus before and after 1000 g/ml treatment. The results show that turmeric nanoparticles limit biofilm gene expression by half. Fold change decreases from 1.06 to 0.46, p-value = 0.015, but Ica D is not significantly affected, and gene expression decreases (1.06-0.77).