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416302

Klebsiella pneumoniae: a Major Pathogen Causing Ventilator Associated Pneumonia at Suez Canal University Hospitals

Article

Last updated: 09 Mar 2025

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Subjects

-

Tags

Clinical microbiology

Abstract

Background: Klebsiella Pneumoniae (K. pneumoniae) is a serious emerging challenge among mechanically ventilated patients due to its disease severity, high virulence and antimicrobial resistance. Genotyping of such an aggressive pathogen is critical tool in restricting Klebsiella infections. Objective: This study aimed to characterize the diversity of Klebsiella pneumoniae complex (KPC) isolates causing ventilator associated pneumonia (VAP) for further reducing morbidity and mortality rates of intensive care unit (ICU) patients. Methodology: K. pneumoniae spp. were isolated from endotracheal tube aspirate specimens of patients admitted to ICU at Suez Canal University Hospitals (SCUHs). Species identification was done using conventional microbiological methods. Antimicrobial susceptibility testing was performed by disk diffusion method. Confirmation of K. pneumoniae was done by PCR amplification of gyrA gene. Genotyping was done by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method using TaqI and HaeIII restriction enzymes. Results: Out of 83 specimens, 32 Enterobacteriaceae strains were isolated, 65.6% were identified to be K. pneumoniae. PCR-RFLP identified 17 isolates(81%) as KPI, 3 (14.3%) as KPII and 1 (4.7%) as KPIII. High rates of MDR and XDR were reported. Among KPI, 8 isolates (47.1%) showed MDR and 8 isolates (47.1%) showed XDR. For KPII, 2 isolates (66.7%) showed MDR and 1(33.3%) showed XDR. The only KPIII isolate was XDR. Conclusion: GyrA PCR-RFLP provides a reliable tool for genotyping of KPC isolates. MDR and XDR KPC isolates are common causative pathogens for VAP at SCUHs and can initiate future outbreaks of nosocomial infections which obligates strengthening the infection control measures to combat such aggressive infections.

DOI

10.21608/ejmm.2025.364044.1497

Keywords

Klebsiella pneumoniae complex, VAP, PCR, RFLP, restriction enzymes

Authors

First Name

Maha

Last Name

Mahdi

MiddleName

M.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt

Email

maha.mohamed@med.suez.edu.eg

City

Ismailia

Orcid

-

First Name

Waheed

Last Name

Hessam

MiddleName

F.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt

Email

w_hessam@hotmail.com

City

Ismailia

Orcid

-

First Name

Atef

Last Name

Mohammed

MiddleName

S.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt

Email

atef.shehata@med.suez.edu.eg

City

Ismailia

Orcid

-

First Name

Yara

Last Name

Marei

MiddleName

E.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt

Email

yarayara253@hotmail.com

City

Ismailia

Orcid

-

First Name

Marwa

Last Name

Fouad

MiddleName

M.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt

Email

marwafouad80@gmail.com

City

Ismailia

Orcid

-

Volume

34

Article Issue

3

Related Issue

53640

Issue Date

2025-07-01

Receive Date

2025-03-02

Publish Date

2025-07-01

Print ISSN

1110-2179

Online ISSN

2537-0979

Link

https://ejmm.journals.ekb.eg/article_416302.html

Detail API

http://journals.ekb.eg?_action=service&article_code=416302

Order

416,302

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Klebsiella pneumoniae: a Major Pathogen Causing Ventilator Associated Pneumonia at Suez Canal University Hospitals

Details

Type

Article

Created At

09 Mar 2025