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P53 Gene Expression, Antibiogram Profile of Staphylococcus aureus and Hormonal Modulation in Acne Patients Treated with Isotretinoin

Article

Last updated: 09 Mar 2025

OverviewSimilar Items

Subjects

-

Tags

Clinical microbiology

Abstract

Background: Acne is a complex skin disorder caused by hormonal changes, bacterial infections and the genetic background. Staphylococcus aureus, cause inflammatory reactions in acne patients. Sebaceous gland hyperactivity and inflammation are intensified by hormonal regulation (particularly high free testosterone levels). P53 is a key mediator of sebocyte death and inflammation. Objectives: The aims of the study are to detect the involvement of Staphylococcus aureus, and hormonal dysregulation in acne patients, and to evaluate the p53 gene expression, as a relation between acne patients and healthy controls, and evaluates therapeutic effects of isotretinoin on the p53 gene expression in acne patients. Methodology:  S. aureus were detected by the routine bacteriology methods. The hormone tests evaluated free testosterone by ELISA kit. The qRT-PCR of the P53 gene expression was measured both before and after administration of isotretinoin. The statistical significance of the results were carried using t-tests; p<0.05, and one-way ANOVA; p<0.05. Results:  Staphylococcus aureus was identified in 35 % of acne patients and 15 % of healthy volunteers, where it was especially resistant to antibiotics showed 40% resistance to clindamycin and 35% to fusidic acid. Free testosterone level increased in acne patients (634.69±83.92 ng/dL) compared with healthy control subjects (469.50±66.55 ng/dL, p=0.001). The gene expression of P53 were much less in patients who didn't receive isotretinoin treatment. Conclusion: Free testosterone is high in acne patients and makes sebaceous glands reactiv, while isotretinoin increases p53 signaling in a therapeutically effective way. Future treatments is required to resistant S. aureus.

DOI

10.21608/ejmm.2025.358496.1468

Keywords

Acne drug, P53 gene expression, Isotretinoin, Staphylococcus aureus, free testosterone

Authors

First Name

Inaam

Last Name

Abed

MiddleName

A.

Affiliation

Department of Medical Microbiology, College of Medicine, University of Babylon, Babylon, Iraq.

Email

inaambed111@gmail.com

City

najaf

Orcid

-

First Name

Mohammed

Last Name

Abdul-Razaq

MiddleName

S.

Affiliation

Department of Medical Microbiology, College of Medicine, University of Babylon, Babylon, Iraq.

Email

mohammedabdu@yahoo.com

City

najaf

Orcid

-

First Name

Wisam

Last Name

Ameen

MiddleName

A.

Affiliation

Department of Dermatology, College of Medicine, University of Babylon, Iraq

Email

wisamewad3@yahoo.com

City

najaf

Orcid

-

Volume

34

Article Issue

3

Related Issue

53640

Issue Date

2025-07-01

Receive Date

2025-02-22

Publish Date

2025-07-01

Print ISSN

1110-2179

Online ISSN

2537-0979

Link

https://ejmm.journals.ekb.eg/article_416265.html

Detail API

http://journals.ekb.eg?_action=service&article_code=416265

Order

416,265

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

P53 Gene Expression, Antibiogram Profile of Staphylococcus aureus and Hormonal Modulation in Acne Patients Treated with Isotretinoin

Details

Type

Article

Created At

09 Mar 2025