Subjects
-Abstract
Background: Acne vulgaris is an inflammatory skin disease which is genetically and immunologically determined. Transcriptor FOXO1 controls oxidative stress and sebocyte differentiation, while cytokines (IL-35 and TRAIL) regulate the immune responses in acne. Objectives: The aim of our work is to detect the modification of FOXO1 gene expression and cytokine levels (IL-35 and TRAIL) in acne vulgaris patients before and after isotretinoin therapy for disease control. Methodology: This case-control study involved 85 individuals in 15–35-year olds. They were divided into three groups pre isotretinoin treatment (n=30), post isotretinion treatment (n=30) and healthy controls (n=25). Serum cytokine concentrations of (IL-35 and TRAIL) were measured by ELISA and FOXO1 mRNA levels were determined by RT-qPCR. Statistical analyses assessed treatment-induced changes. Results: FOXO1 mRNA was significantly down regulated in untreated acne patients as compared with controls (p=0.001). Treatment with isotretinoin increased FOXO1 expression 2.7-fold. In the untreated patients, IL-35 and TRAIL levels were both suppressed. IL-35 and TRAIL significantly (p<0.05) increased in comparison with controls after treating with Isotretinion. Conclusion: This FOXO1 and cytokines (IL-35 and TRAIL) activity is modulated by isotretinoin which further emphasizes their involvement in the acne pathogenesis and response. These results highlight FOXO1 and cytokine profiling as biomarkers for treatment efficacy and as targets for novel therapy in acne vulgaris.
DOI
10.21608/ejmm.2025.358449.1466
Keywords
Acne vulgaris, FoxO1, Isotretinoin, Il-35, TRAIL
Authors
Affiliation
Department of Medical Microbiology, College of Medicine, University of Babylon, Babylon, Iraq.
Email
inaambed111@gmail.com
Orcid
-Affiliation
Department of Medical Microbiology, College of Medicine, University of Babylon, Babylon, Iraq.
Email
mohammedabdu@yahoo.com
Orcid
-Affiliation
Department of Dermatology, College of Medicine, University of Babylon, Iraq.
Email
wisamewad3@yahoo.com
Orcid
-Link
https://ejmm.journals.ekb.eg/article_416262.html
Detail API
http://journals.ekb.eg?_action=service&article_code=416262
Type
New and original researches in the field of Microbiology.
Publication Title
Egyptian Journal of Medical Microbiology
Publication Link
https://ejmm.journals.ekb.eg/
MainTitle
The Impact of FOXO1 Gene Expression and Cytokine Profiling in Acne Patients Treated with Isotretinoin