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415050

Cross Resistance against some Microbicides Exposure in Clinically MDR Pseudomonas aeruginosa isolates from Iraqi Patients

Article

Last updated: 09 Mar 2025

OverviewSimilar Items

Subjects

-

Tags

Genetics

Abstract

Background: Microbicide resistance poses a severe danger to the protection of life, health, and the prudent use of resources in order to prevent the spread of nosocomial infections. Objectives: This study aims to investigate the linkages between tolerance towards microbicides and induction of antibiotics resistance, in addition to evaluate the expression response of efflux pump qacE∆1 gene in clinically MDR Pseudomonas aeruginosa isolates. Methodology: The susceptibility of 38 identified clinical P. aeruginosa isolates to certain microbicides (benzalkonium chloride and chlorhexidine digluconate) was examined in addition to determine the minimum inhibitory concentration (MIC) of these agents. Furthermore, the presence and expression level of qacE∆1 gene was measured using PCR and RT-PCR. Results: The results indicated that the MIC of benzalkonium chloride ranged from 128-8µg/ml while for chlorhexidine digluconate ranged from 512-128 µg/ml. The presence of efflux pump encoded gene qacE∆1 was identified in (89.47%) of examined isolates. Quantifying the expression of qacE∆1 gene showed significantly increased in fold change upon treated with Chlorhexidine digluconate and Benzalkonium chloride about (3.953 ±0.99) and (3.783 ±0.71) respectively. Conclusion: Due to widespread use of microbicides in hospitals, concerns about the emergence and transmission of microbicide resistance genes have raised which induce the resistance antibiotic in pathogenic P. aeruginosa. In addition, a crucial correlation between the investigated microbicide and the efflux pump-encoded gene qacE∆1.

DOI

10.21608/ejmm.2025.360914.1478

Keywords

Pseudomonas aeruginosa, efflux pump, qacE∆1, Microbicides, Cross resistance

Authors

First Name

Donya

Last Name

Ahmed

MiddleName

A.

Affiliation

Institute of Genetic Engineering and Biotechnology for Postgraduate Studies, University of Baghdad, Baghdad- Iraq

Email

donya.ayad@yahoo.com

City

-

Orcid

-

First Name

Abdulameer

Last Name

Ghareeb

MiddleName

M.

Affiliation

Institute of Genetic Engineering and Biotechnology for Postgraduate Studies, University of Baghdad, Baghdad- Iraq

Email

-

City

-

Orcid

-

Volume

34

Article Issue

3

Related Issue

53640

Issue Date

2025-07-01

Receive Date

2025-02-15

Publish Date

2025-07-01

Print ISSN

1110-2179

Online ISSN

2537-0979

Link

https://ejmm.journals.ekb.eg/article_415050.html

Detail API

http://journals.ekb.eg?_action=service&article_code=415050

Order

415,050

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Cross Resistance against some Microbicides Exposure in Clinically MDR Pseudomonas aeruginosa isolates from Iraqi Patients

Details

Type

Article

Created At

09 Mar 2025