Beta
ManageChatDiscover
415049

Synthesis of Chitosan Collagen Nanoparticles from Iraqi Catfish (Siluriformes) Skin and Evaluation of their Antibacterial Activity

Article

Last updated: 09 Mar 2025

OverviewSimilar Items

Subjects

-

Tags

Antimicrobial agents

Abstract

Background: Chitosan-collagen nanoparticles have emerged as promising materials for biomedical applications due to their excellent biocompatibility, biodegradability, and antimicrobial properties.  The synthesis of nanocollagen from Iraqi catfish (Siluriformes) skin using chitosan and sodium tripolyphosphate (STPP) as crosslinking agents represents an innovative approach to creating biocompatible nanoparticles. The study utilizes various analytical techniques such as FTIR, UV-Vis, XRD, and SEM to characterize and understand the properties of nanoparticles. Objective: This study aimed to evaluate the efficacy of nano-collagen in managing infections caused by multidrug-resistant bacteria such as Staphylococcus aureus and Pseudomonas aeruginosa. Methodology: Bacterial isolates were collected from patients with wound infections at the Al-Zahrawi Hospital in Maysan. Identification was confirmed using selective media (MacConkey agar and mannitol salt agar) and standard microbiological tests, including Gram staining and biochemical assays. The Vitek-2 system was used for precise bacterial identification. Results: Minimum inhibitory concentration (MIC) tests demonstrated that nano-collagen doubled the antibacterial efficacy of collagen extract. The MIC was 47.5 µg/mL (nano-collagen) vs.  95 µg/mL (collagen extract).  against S. aureus the MIC was 95 µg/mL (nano-collagen) vs. 380 µg/mL (collagen extract). Conclusion: These findings suggest that nanocollagen is a promising biomaterial for regenerative medicine and advanced wound care.

DOI

10.21608/ejmm.2025.360748.1476

Keywords

collagen, Nanocollagen, Chitosan, FTIR, XRD

Authors

First Name

Dooa

Last Name

Jebur

MiddleName

M.

Affiliation

Institute of genetic engineering and Biotechnology for Higher Studies, University of Baghdad, Iraq

Email

mdidy00@gmail.com

City

-

Orcid

0009-0008-1812-4219

First Name

Shurook

Last Name

Saadedin

MiddleName

M.K.

Affiliation

Institute of genetic engineering and Biotechnology for Higher Studies, University of Baghdad, Iraq

Email

ssaadedin@gmail.com

City

-

Orcid

0000-0002-0386-4328

Volume

34

Article Issue

3

Related Issue

53640

Issue Date

2025-07-01

Receive Date

2025-02-15

Publish Date

2025-07-01

Print ISSN

1110-2179

Online ISSN

2537-0979

Link

https://ejmm.journals.ekb.eg/article_415049.html

Detail API

http://journals.ekb.eg?_action=service&article_code=415049

Order

415,049

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Synthesis of Chitosan Collagen Nanoparticles from Iraqi Catfish (Siluriformes) Skin and Evaluation of their Antibacterial Activity

Details

Type

Article

Created At

09 Mar 2025