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415048

Detection of Chlamydia trachomatis in Women Experiencing Miscarriage and Molecular Insights in AL-Anbar Region

Article

Last updated: 09 Mar 2025

OverviewSimilar Items

Subjects

-

Tags

Diagnostics

Abstract

Background: Chlamydia trachomatis is the primary cause of many sexually transmitted diseases. This infection is widespread globally and can last a long time, traveling upward through the genital system. This quiet infection within cells is linked to harmful effects on pregnancy, such as the risk of miscarriage. Objective: Evaluation of gene expression of Chlamydia trachomatous among women who experienced miscarriage and seronegative for the bacteria. Methodology: The research was conducted at several hospitals and laboratories between December 2023 and August 2024. 55 patients and 110 controls who had undergone previous legal abortion due to intrauterine death were included in the study. Vaginal swabs were taken from each case and analyzed using Chlamydia Antigen ELISA technique, and PCR for Chlamydia trachomatis. Results: All patients were seronegative for Chlamydia trachomatis. The successful amplification of the Chlamydia trachomatis gene through multiplex conventional PCR was considered a favorable result for PCR Chlamydia trachomatis. Among the 55 DNA samples taken from vaginal samples, zero was positive for OMP A, 30 (54.55%) were positive for CPAF gene, and 33 (60%) were positive for TOX gene, 25 (45%) were positive for Tarp gene indicating the presence of Chlamydia trachomatis p-value < 0.001 Conclusion:  Polymerase chain reaction has proven to be a more effective and efficient method for diagnosing Chlamydia using nucleic acid amplification tests. Our findings suggest a connection between Chlamydia trachomatis infection and miscarriage, underlining the importance of early detection and treatment of this infection in pregnant individuals to potentially prevent miscarriages.

DOI

10.21608/ejmm.2025.359999.1474

Keywords

Miscarriages, Chlamydia Trachomatis, genes identification, vaginal, Bacteria

Authors

First Name

Rasha

Last Name

Rajab

MiddleName

K.

Affiliation

Medical Microbiology Department, College of Medicine, University of Anbar, Ramadi, Iraq

Email

ras21m0004@uoanbar.edu.iq

City

-

Orcid

-

First Name

Yasir

Last Name

Abdulateef

MiddleName

M

Affiliation

Medical Microbiology Department, College of Medicine, University of Anbar, Ramadi, Iraq

Email

dr.yasserjanai@uoanbar.edu.iq

City

Ramadi

Orcid

-

First Name

Sawsan

Last Name

Al-Quhli

MiddleName

Q.T.

Affiliation

Medical Microbiology Department, College of Medicine, University of Anbar, Ramadi, Iraq

Email

sawsanqt@uoanbar.edu.iq

City

Ramadi

Orcid

-

Volume

34

Article Issue

3

Related Issue

53640

Issue Date

2025-07-01

Receive Date

2025-02-12

Publish Date

2025-07-01

Print ISSN

1110-2179

Online ISSN

2537-0979

Link

https://ejmm.journals.ekb.eg/article_415048.html

Detail API

http://journals.ekb.eg?_action=service&article_code=415048

Order

415,048

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Detection of Chlamydia trachomatis in Women Experiencing Miscarriage and Molecular Insights in AL-Anbar Region

Details

Type

Article

Created At

09 Mar 2025