Background: Integrating generative Artificial Intelligence (AI) technology into practical training of nursing students represents a notable evolution in nursing education. Aim: to assess knowledge of nursing students regarding artificial intelligence and its impact on practical training at the faculty of nursing Beni-suef university. Research design: A descriptive research design was applied in this study Setting: This study was conducted in Faculty of Nursing at Beni- Suef city, Egypt. Sample: simple random sample was equal 255 nursing students at fourth year students at Faculty of Nursing at Beni -Suef city. Tools for Data Collection:. One tool ,Interviewing Questionnaire Sheet, comprised of four parts. the four parts were : personal characteristic ,family data ,knowledge of nursing students regarding AI and Impact of using Artificial intelligence on practical training Result:. The study showed that 48.6% of the studied students had a good level of knowledge regarding artificial intelligence and 63.1% of them had high perceived impact of artificial intelligence on practical training. Conclusion: there was highly statistically significant positive association between the studied participants' total AI knowledge score and their perceived impact of artificial intelligence on practical training. and there was a highly statistical significant relation between total AI knowledge and their age , mother education , number of family member , monthly family income, and there was a highly statistical significant relation between total perceived impact of artificial intelligence on practical training and their mother's education . Recommendation : Regular training program for university students to improve their knowledge about artificial intelligence.