Background: Diabetes mellitus is a serious condition that affects lipoprotein metabolism and fetal growth. Diabetic mothers' lipid profiles can change, resulting in multi-system problems and influencing their prognosis. This study aimed to measure serum lipid profile in infant of diabetic mothers and their mothers and assesses their relations to Infant of diabetic mother (IDM) complications if present. Methods: This study was case control study which conducted in the neonatology unit of Pediatrics Department at Zagazig University children Hospital and Obstetric Departments. It was conducted on 30 infants (15 IDM and 15 normal babies included as a control group) and their mothers (15 IDM mothers and 15 normal mothers). All patients had a thorough history-taking process for mothers as well as a full clinical examination, Laboratory investigations for both mothers and neonatal (case and control groups) included Serum lipid profile, Triglycerides, Cholesterol. Results: There was statistical significance increase in mean weight of infants of diabetic mothers compared to infants of normal mothers. There was increase in mean LDL, Cholesterol and TG among diabetic mothers compared to normal mothers but with no statistical significance differences. There was a statistical significance increase in low density lipoprotein (LDL), cholesterol and triglyceride of infants of diabetic mothers compared to infants of normal mothers.. Conclusion: Serum cholesterol, triglycerides and LDL levels are higher in diabetic mothers that may be reflected on the metabolism and growth of the fetus