The composition, number, and community structure of zooplankton, along with important physical and chemical parameters, were used to assess the state of the Nile water in Upper Egypt between Aswan and Sebaiyia during 2021. The study was conducted in the south 120km of the Nile's main stream in Upper Egypt, downstream of the Aswan Old Dam (between 24°04′ and 25°00′ latitudes and 32°51′ and 32°54′ longitudes). The three taxonomic groupings that comprised the bulk of zooplankton species were Rotifera (23 species), Copepoda (3 species), and Cladocera (5 species).  One species of Platyhelminthes was among the other uncommon zooplankton types that were sometimes observed. The community structure of zooplankton was classified based on temperature (Cº), conductivity (EC), pH, dissolved oxygen (DO), nitrite (NO₂), nitrate (NO₃), orthophosphate (PO₄), organic matter (OM), carbonate (CO₃), and chlorophyll-a using canonical corresponding analysis (CCA). According to the zooplankton's documented temporal trend, they peaked in the springtime when phytoplankton bloomed. Otherwise, a relatively highwater flow rate coincided with the low zooplankton abundance throughout the summer-fall period, which may potentially be considered another abiotic factor affecting zooplankton development. Furthermore, the west bank locations had twice as many as the east sites that were directly exposed to industrial wastes. This suggests that wastewater discharge limited the abundance of the Nile zooplankton assemblages, primarily because rotifers and cladocerans were declining in number.