The studies seeds are good sources of total crude lipids (30.71%- 39.70%) and crude proteins. The qualitative screening
of active secondary metabolites in individual oils and Canochia revealed the presence of 12 groups of bioactive compounds. The physicochemical properties of CSO, Chia-SO, and binary blend Canochia which composed newly with a ratio of 50:50%. These results are considered a positive indicator for oil blending. The highest level of ω-3 was in Chia-SO, followed by Canochia, and CSO was superior in its content of ω-9, as its concentration reached 70.23%. The concentrations of 12 fatty acids found in CSO, Chia-SO and Canochia were assayed. There are correlations between fatty acid composition and health lipid indices of chosen oils of plant seeds. Results of atherogenicity index, thrombogenicity index and Hypocholesterolimic /Hypercholesterolemic were calculated. Canola seeds superior in the concentrations of total phenolic compounds (2989.8 mg/100 GAE) followed by CSO (747.9) while Chia-SO ranked the lowest position (91.45). The total carotenoids and total chlorophylls in the raw seeds are always higher than in the oils. The highest values of DPPH-RSA% are found in canola seed extract (57.19%) followed by Chia-seed (38.43%). The OSI of 3 oil samples i.e. CSO, Chia-SO and Canochia are evaluated based on induction period (IP) measurement determined by Rancimat analysis. The individual pure canola oil has the highest OSI (18.77±0.19) among other pure oils. The binary blend Canochia have induction period 12.21±0.13. The OSI of the oil samples is ordered as CSO > Canochia > Chia-SO.