Acetamiprid (ACE) is one of the most worldwide used neonicotinoids. The current study aimed at investigating the protective effects of vitamin E (Vit E) (100 IU/kg/day) and/or Coenzyme Q10 (CoQ10) (75 mg/kg/day) against (ACE) (25 mg/kg/day) induced male reproductive toxicity. Eight groups of adult male Sprague Dawley rats (n=6) were used as the following: Control, oil, (Vit E), (CoQ10), (ACE), (ACE + Vit E), (ACE+CoQ10) and (ACE+ Vit E+CoQ10). Rats received treatments by oral gavage for 60 days. The administration of (ACE) showed significantly decreased of the following: (body weights, testicular weights, serum testosterone, serum luteinizing hormone, testicular antioxidant reduced glutathione, testicular superoxide dismutase activity, sperms count and percentage of sperms motility); in line with significantly increased of the following: (percentage of dead sperms, percentage of abnormal sperms and testicular malondialdehyde). Histologically; (ACE) group showed marked degenerative changes in the seminiferous tubules with increased immune histochemical expression of testicular (Caspase-3 and Tumor necrotic factor alpha). Separate administration of either (Vit E) or (CoQ10) with (ACE) showed improvement of ACE induced histological, oxidative, inflammatory, apoptotic, semen toxic effects, but failed to improve serum testosterone, body and testicular weights. Moreover, combined protective effects of (Vit E) and (CoQ10) significantly overcame the separate use of both agents in improving body and testicular weights, sperm motility, vitality, testosterone, luteinizing hormone and Caspase-3 expression. Finally, the administration of either (Vit E) or (CoQ10) showed to have protective effects against (ACE) induced male reproductive toxicity, although the combined use of both agents was more effective.