As a prevalence disease, breast cancer studies focus on the variations occurring with genes involved on the carcinogenesis. The CYP1A1 one of the most important cytochromes in (CYP1) family, which is exists at most in tissues (extrahepatic) and contributing in the metabolism process of a wide kinds of the xenobiotics, as well as many of endogenous substrates.  Cytochrome CYP1A1 catalyzes several of reactions, but there is a unique reaction, hydroxylation of an aromatic ring at an unoccupied position producing of high reactive species that can cause carcinogenic mutations, considered to be the hallmark of CYP1A1 to initiate the carcinogenesis. so that one of the most interesting genes was CYP1A1.The current study was included 100 patients affected with breast cancer and others 100 healthy women as control group, where aimed to evaluating the role of (CYP 1A1) gene polymorphisms and the association with the risk of breast cancer among Egyptian women and its relation with biochemical investigations, hematological parameters, and tumor marker.